The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 28, 2015

Filed:

May. 04, 2009
Applicants:

Jerome J. Jendrisak, Madison, WI (US);

Ramesh Vaidyanathan, Madison, WI (US);

Ronald Meis, Fitchburg, WI (US);

Inventors:

Jerome J. Jendrisak, Madison, WI (US);

Ramesh Vaidyanathan, Madison, WI (US);

Ronald Meis, Fitchburg, WI (US);

Assignee:

CellScript, LLC, Madison, WI (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01); G01N 33/00 (2006.01); C07H 21/02 (2006.01); C12Q 1/42 (2006.01); C12N 9/14 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/42 (2013.01); C12N 9/14 (2013.01); C12P 19/34 (2013.01);
Abstract

The present invention relates to the discovery of RNA 5' polyphosphatase enzymes not previously described in the art, methods for discovery of said enzymes, compositions of said enzymes, methods for making said enzymes, and various methods and kits for using said enzymes for biomedical research, for human and non-human diagnostics, for production of therapeutic products, and for other applications. In particular, some embodiments provide compositions, kits and methods for employing RNA polyphosphatases for isolation, purification, production, and assay of capped RNA using a biological sample or a sample from an in vitro capping reaction wherein the sample also contains RNA that is not capped. Other embodiments provide compositions, kits and methods wherein RNA polyphosphatases comprise signal-amplifying enzymes for analyte-specific assays.


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