The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 28, 2015

Filed:

Feb. 03, 2010
Applicants:

Yingfang Liu, Beijing, CN;

Zihe Rao, Beijing, CN;

Inventors:

Yingfang Liu, Beijing, CN;

Zihe Rao, Beijing, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/34 (2006.01); C07K 14/005 (2006.01); C12N 9/12 (2006.01); C40B 30/02 (2006.01); G06F 19/16 (2011.01); G06F 19/00 (2011.01);
U.S. Cl.
CPC ...
C07K 14/005 (2013.01); C07K 2299/00 (2013.01); C12N 9/127 (2013.01); C12N 2760/16122 (2013.01); C12N 2760/16222 (2013.01); C12N 2760/16322 (2013.01); C40B 30/02 (2013.01); G06F 19/16 (2013.01); G06F 19/706 (2013.01);
Abstract

Present invention discloses the three-dimensional crystal structure of the N-terminus polypeptide of influenza virus polymerase subunit (PA_N of SEQ ID NO:7). PA_N of SEQ ID NO: 1 is residues 1—50 to 150-300 of influenza virus polymerase subunit PA. In the three-dimensional structure, at least 40% of atoms show the same atomic coordinates, compared to that listed in Table 1. Namely, in the three-dimensional structure of influenza virus polymerase subunit PA_N of SEQ ID NO: 7, 40% of atomic coordinates on carbon skeleton of residues of influenza virus polymerase subunit PA_N of SEQ ID NO: 7, show less than or equal to 1.7 Å of average variance, compared to the atomic coordinates listed in Table 1. Present invention also discloses the expression, purification, crystallization methods, and three-dimensional crystal structure of 256 residues in the N-terminus of influenza virus polymerase subunit PA, and applications of the crystal structure of SEQ ID NO: 7 on drug screening and designing.


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