The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 28, 2015

Filed:

Mar. 16, 2012
Applicant:

Ji Hoon Lee, Gaithersburg, MD (US);

Inventor:

Ji Hoon Lee, Gaithersburg, MD (US);

Assignee:

Luminescent MD, LLC, , MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); G01N 33/533 (2006.01); C12Q 1/28 (2006.01); G01N 33/535 (2006.01); G01N 33/542 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5306 (2013.01); C12Q 1/28 (2013.01); G01N 33/535 (2013.01); G01N 33/542 (2013.01);
Abstract

Additives capable of controlling the reaction rate between substrate and enzyme to form a dye in enzyme assays with absorbance, chemiluminescence, and fluorescence are effective tools for optimizing enzyme assays. A dye can be formed rapidly from the reaction between enzyme and substrate in the presence of a catalyst. Using relatively high concentration of dye formed from the rapid reaction, trace levels of analytical materials can be quantified using absorbance, chemiluminecence and fluorescence detection. A dye can be formed from a relatively slow reaction between enzyme and substrate in the presence of a surfactant such as Triton X-100 and β-cyclodextrin. Using relatively low concentration of dye from the slow reaction, a high concentration of analytical material can be quantified without any dilution using absorbance, chemiluminescence, and fluorescence.


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Lee, Ji. (2015). Method for enhancing enzyme assays (U.S. Patent No. 9017950). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/9017950

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