The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 21, 2015

Filed:

Oct. 05, 2011
Applicants:

Ton Logtenberg, Driebergen, NL;

Hendricus Renerus Jacobus Mattheus Hoogenboom, Maastricht, NL;

Inventors:
Assignee:

Merus B.V., Utrecht, NL;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C40B 30/04 (2006.01); C12N 15/10 (2006.01); C07K 16/10 (2006.01); C07K 16/20 (2006.01); C07K 16/40 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1086 (2013.01); C12N 15/1082 (2013.01); C07K 16/10 (2013.01); C07K 16/20 (2013.01); C07K 16/40 (2013.01); C07K 2317/22 (2013.01); C07K 2317/569 (2013.01); C07K 2318/20 (2013.01);
Abstract

Described are methods for producing libraries of cells expressing at least two separate single polypeptide chain binding proteins, in which the binding proteins have different target epitopes. Such libraries are made by integration of the nucleic acid sequences encoding the polypeptide chains into the genome of the host cell, and selecting for cells that have successfully integrated these nucleic acids. The selected cells are preferably subjected to a cloning step. Mixtures of binding proteins are produced without having to individually produce each of the components of the mixture. A library of cells wherein essentially each cell encodes at least two single polypeptide chain binding proteins having different target epitopes is also herewith provided, as well as methods for producing a composition comprising at least two separate single polypeptide chain binding proteins having different target epitopes.


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