The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 21, 2015

Filed:

Jul. 06, 2005
Applicant:

Roberto Crea, Foster City, CA (US);

Inventor:

Roberto Crea, Foster City, CA (US);

Assignee:

Pfizer Inc., New York, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C40B 30/04 (2006.01); C12N 15/10 (2006.01); C07K 16/00 (2006.01); C07K 16/18 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
C12N 15/102 (2013.01); C07K 16/00 (2013.01); C07K 16/18 (2013.01); C07K 2317/565 (2013.01); C07K 2317/622 (2013.01); C07K 2317/92 (2013.01); C12N 15/1093 (2013.01); G01N 33/6854 (2013.01); G01N 33/6857 (2013.01);
Abstract

A method of mutagenesis by which a predetermined amino acid is introduced into each and every position of a selected set of positions in a preselected region (or several different regions) of a polypeptide to produce a library of polypeptide analogs is disclosed. The method is based on the premise that certain amino acids play a crucial role in the structure and function of proteins and thus is capable of identifying and distinguishing functional amino acid residues ('hot spots') from non-functional amino acids residues ('cold spots') within a polypeptide or portion thereof. Libraries can be generated which contain only desired polypeptide analogs and are of reasonable size for screening. The libraries can be used to study the role of specific amino acids in polypeptide structure and function and to develop new or improved polypeptides such as antibodies, antibody fragments, single chain antibodies, enzymes, and ligands.


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