The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 21, 2015

Filed:

May. 05, 2010
Applicants:

Kurt Vagle, Longmont, CO (US);

William S. Marshall, Boulder, CO (US);

Inventors:

Kurt Vagle, Longmont, CO (US);

William S. Marshall, Boulder, CO (US);

Assignee:

miRagen Therapeutics, Boulder, CO (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 9/127 (2006.01); A61K 31/7105 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); C07J 41/00 (2006.01); C07J 43/00 (2006.01); C07J 51/00 (2006.01); C12N 15/87 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01); A61K 48/00 (2006.01);
U.S. Cl.
CPC ...
C07J 41/0055 (2013.01); C07J 43/003 (2013.01); C07J 51/00 (2013.01); C12N 15/87 (2013.01); C12N 15/111 (2013.01); C12N 15/113 (2013.01); A61K 48/0033 (2013.01); C12N 2310/113 (2013.01); C12N 2310/315 (2013.01); C12N 2310/3515 (2013.01);
Abstract

Disclosed are lipophilic polynucleotide conjugates including polynucleotide-cholesterol conjugates and methods of delivering therapeutic polynucleotides to a mammalian cell or patient in need of treatment using said conjugates. The disclosure further provides methods of synthesizing the lipophilic polynucleotide conjugates. The conjugates are designed to mimic or target cellular miRNAs. The lipophilic moiety, such as cholesterol or cholesterol derivative, is spaced from the polynucleotide by a substantially linear hydrocarbon group. Due to an absence of significantly polar groups and/or exchangeable protons in the vicinity of the lipophilic moiety, the interaction between the lipophilic moiety and cell membranes is enhanced to provide for efficient entry into cells.


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