The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 21, 2015

Filed:

Apr. 15, 2011
Applicants:

Norikazu Nishino, Fukuoka, JP;

Yasushi Takemoto, Saitama, JP;

Akihiro Ito, Saitama, JP;

Minoru Yoshida, Tokyo, JP;

Inventors:

Norikazu Nishino, Fukuoka, JP;

Yasushi Takemoto, Saitama, JP;

Akihiro Ito, Saitama, JP;

Minoru Yoshida, Tokyo, JP;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 21/78 (2006.01); C12Q 1/37 (2006.01); C12Q 1/48 (2006.01); G01N 33/68 (2006.01); C07D 311/18 (2006.01); C07D 311/82 (2006.01); C07D 473/32 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/37 (2013.01); C12Q 1/48 (2013.01); G01N 2500/00 (2013.01); G01N 33/6875 (2013.01); C07D 311/18 (2013.01); C07D 311/82 (2013.01); C07D 473/32 (2013.01);
Abstract

Provided are a method for measuring histone methyltransferase activity, a method for screening for compounds that inhibit histone methyltransferase activity, a reagent kit for measuring histone methyltransferase activity, and a kit for screening for compounds that inhibit histone methyltransferase activity. A substrate compound represented by general formula (I): R-X-K-R(I), or a salt thereof, wherein Rrepresents a hydrogen atom or a protecting group for an amino terminus; X represents a peptide consisting of 0 or 1 or more amino acid residues; K represents a lysine residue; and Rrepresents a dye label linked via an amide bond to the carbonyl terminus of a lysine residue, wherein the cleavage of the amide bond by peptidase changes the fluorescence property or chromogenic property of the dye label, and the methylation of the ε amino group of the lysine residue by the histone methyltransferase decreases susceptibility to peptidase, is used.


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