The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 21, 2015

Filed:

Jul. 03, 2007
Applicants:

Tzyy-choou Wu, Stevenson, MD (US);

Chien-fu Hung, Timonium, MD (US);

Inventors:

Tzyy-Choou Wu, Stevenson, MD (US);

Chien-Fu Hung, Timonium, MD (US);

Assignee:

The Johns Hopkins University, Baltimore, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 38/00 (2006.01); C07H 21/04 (2006.01); C12N 15/11 (2006.01); A61K 31/7088 (2006.01); C12N 15/113 (2010.01);
U.S. Cl.
CPC ...
C12N 15/111 (2013.01); A61K 31/7088 (2013.01); C12N 15/113 (2013.01); C12N 2310/14 (2013.01); C12N 2320/31 (2013.01);
Abstract

An immunotherapeutic strategy is disclosed that combines antigen-encoding DNA vaccine compositions combined with siRNA directed to pro-apoptotic genes, primarily Bak and Bax, the products of which are known to lead to apoptotic death. Gene gun delivery (particle bombardment) of siRNA specific for Bak and/or Bax to antigen-expressing DCs prolongs the lives of such DCs and lead to enhanced generation of antigen-specific CD8+ T cell-mediated immune responses in vivo. Similarly, antigen-loaded DC's transfected with siRNA targeting Bak and/or Bax serve as improved immunogens and tumor immunotherapeutic agents.


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