The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 14, 2015

Filed:

Aug. 16, 2011
Applicants:

Christian Korfhage, Langenfeld, DE;

Gerd Grosshauser, Pulheim, DE;

Thomas Rothmann, Langenfeld, DE;

Ralf Himmelreich, Langenfeld, DE;

Inventors:

Christian Korfhage, Langenfeld, DE;

Gerd Grosshauser, Pulheim, DE;

Thomas Rothmann, Langenfeld, DE;

Ralf Himmelreich, Langenfeld, DE;

Assignee:

Qiagen GmbH, Hilden, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6844 (2013.01); C12Q 1/6844 (2013.01); C12Q 2521/301 (2013.01); C12Q 2521/513 (2013.01);
Abstract

The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.


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