The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 07, 2015
Filed:
Jan. 29, 2003
Mitali Samaddar, Andhra Pradesh, IN;
Jonathan Michael Blackburn, Cambridge, GB;
Darren James Hart, Grenoble, FR;
Michael Richard Dyson, Great Shelford, GB;
Mitali Samaddar, Andhra Pradesh, IN;
Jonathan Michael Blackburn, Cambridge, GB;
Darren James Hart, Grenoble, FR;
Michael Richard Dyson, Great Shelford, GB;
Sense Proteomic Limited, Oxford, GB;
Abstract
The use of a tag moiety comprising a biotinylation domain, such as biotin carboxyl carrier protein (BCCP), as a protein folding marker and protein solubility enhancer in the orientated surface capture of products of heterologously expressed genes is described. Methods for increasing the solubility of proteins and determining the folded state of a protein are also disclosed. The uses and methods of the invention can be carried out in a multiplexed manner on more than one protein in the formation of libraries. In addition the nucleic acid molecule encoding the biotinylation domain of the tag moiety can be used to increase the proportion of clones in a library that express the protein of interest.