The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 07, 2015

Filed:

Sep. 04, 2012
Applicants:

Pramod K. Gupta, Federal Way, WA (US);

Diane G. Holmstrom, Bonney Lake, WA (US);

Pamela L. Carlson, Federal Way, WA (US);

Inventors:

Pramod K. Gupta, Federal Way, WA (US);

Diane G. Holmstrom, Bonney Lake, WA (US);

Pamela L. Carlson, Federal Way, WA (US);

Assignee:

Weyerhaeuser NR Company, Federal Way, WA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01H 4/00 (2006.01); C12N 5/04 (2006.01);
U.S. Cl.
CPC ...
A01H 4/005 (2013.01); C12N 5/04 (2013.01);
Abstract

The invention provides methods for multiplying conifer embryogenic tissue. The methods of the invention comprise the steps of: (a) culturing conifer embryogenic tissue in or on a solid multiplication medium comprising one or more growth hormones; and (b) culturing the conifer embryogenic tissue multiplied in step (a) in or on a liquid multiplication medium comprising one or more growth hormones, wherein the initial concentration of growth hormones in the solid multiplication medium of step (a) is greater than the initial concentration of growth hormones in the liquid multiplication medium of step (b).


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