The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 31, 2015
Filed:
Sep. 22, 2009
Martin L. Yarmush, Piscataway, NJ (US);
Eric J. Wallenstein, Morris Plains, NJ (US);
Rene S. Schloss, East Brunswick, NJ (US);
Martin L. Yarmush, Piscataway, NJ (US);
Eric J. Wallenstein, Morris Plains, NJ (US);
Rene S. Schloss, East Brunswick, NJ (US);
Rutgers, The State University of New Jersey, New Brunswick, NJ (US);
Abstract
The present invention provides systems and methods for improving the efficiency of a transient gene delivery system to differentiating embryonic stem (ES) cells by serum starving the targeted cells for one to three days prior to transfection. Such a serum starvation surprisingly resulted in increased expression of a constitutively-controlled plasmid from 50.4% to 83.2% of the population and increased expression of a promoter/enhancer controlled plasmid from ˜1.4% to ˜3.7% of the population.