The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 31, 2015
Filed:
Sep. 19, 2013
Applicant:
Thrombogenics NV, Leuven, BE;
Inventor:
Luc Schoonjans, Wilsele, BE;
Assignee:
ThromboGenics N.V., Leuven, BE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/06 (2006.01); C12N 5/0735 (2010.01); A01K 67/027 (2006.01); C07K 14/54 (2006.01); C12N 5/0789 (2010.01); C12N 5/078 (2010.01); A61K 35/12 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0606 (2013.01); C12N 5/0634 (2013.01); C12N 2501/10 (2013.01); C12N 2502/16 (2013.01); A61K 35/51 (2013.01); A01K 67/0275 (2013.01); A01K 2217/05 (2013.01); A61K 2035/124 (2013.01); C07K 14/5415 (2013.01); C12N 5/0647 (2013.01); C12N 2501/2306 (2013.01); C12N 2501/235 (2013.01); C12N 2501/33 (2013.01); C12N 2502/1323 (2013.01); C12N 2502/99 (2013.01);
Abstract
The present invention is directed to a method of deriving pluripotent embryonic stem cells from mouse blastocysts or from primordial germ cells from a post-implantation mouse embryo, or of maintaining or growing pluripotent embryonic stem cells from a mouse, or of expanding human hematopoietic stem cells or human hematopoietic precursor cells. The methods include the step of cultivating the stem cells or precursor cells for at least one passage in a culture medium preconditioned by the rabbit fibroblast cell line Rab9 (ATCC catalogue CRL1414) and containing less than 0.1 ng/ml Leukemia Inhibitory Factor (LIF).