The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 31, 2015

Filed:

May. 24, 2007
Applicants:

Catherine Ronin, Sausset les Pins, FR;

Gaelle Guiraudie-capraz, Lancon de Provence, FR;

Inventors:

Catherine Ronin, Sausset les Pins, FR;

Gaelle Guiraudie-Capraz, Lancon de Provence, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/10 (2006.01); C12P 21/06 (2006.01); C12P 21/04 (2006.01); C12N 9/24 (2006.01); C12N 5/00 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1048 (2013.01); C12N 9/1051 (2013.01); C12N 9/1081 (2013.01); C12Y 204/01087 (2013.01); C12Y 204/01133 (2013.01); C12Y 204/99001 (2013.01); C07K 2319/05 (2013.01);
Abstract

The present invention relates to the production of gene sequences encoding chimerical membrane glycosyltransferases presenting an optimized glycosylation activity in cells transformed with the sequences, the gene sequences corresponding to the fusion: of a first nucleic acid coding for a C-terminal minimal fragment of the catalytic domain (CD) of the native full length glycosyltransferase, to a second nucleic acid coding for a transmembrane peptide comprising in its N-terminal region a cytoplasmic tail (CT) region located upstream from a transmembrane domain (TMD), itself located upstream of a stem region (SR), provided that at least one of these CT, TMD, SR peptides being different from the primary structure of the naturally occurring peptide counterparts present in the native glycosyltransferase from which is derived the CD fragment with optimal glycosyltransferase activity as defined above.


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