The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 24, 2015
Filed:
Feb. 04, 2010
Applicant:
Adrian Salic, Cambridge, MA (US);
Inventor:
Adrian Salic, Cambridge, MA (US);
Assignee:
President and Fellows of Harvard College, Cambridge, MA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/02 (2006.01); C07C 213/00 (2006.01); C07C 215/00 (2006.01); C07C 217/00 (2006.01); C07C 247/00 (2006.01); A61K 49/00 (2006.01); A61K 47/48 (2006.01); G01N 33/92 (2006.01); G01N 1/30 (2006.01);
U.S. Cl.
CPC ...
A61K 49/0041 (2013.01); A61K 47/4813 (2013.01); G01N 1/30 (2013.01); G01N 33/92 (2013.01);
Abstract
The present invention provides a method to label phospholipids in vivo based on the metabolic incorporation of an alkynyl- or azido-labeled metabolic precursor into phospholipids. The resulting phospholipids have alkynyl or azido moieties, which, upon reaction with a labeled azide or alkyne, respectively, form labeled compounds that can be visualized using optical or electron microscopy with high sensitivity and spatial resolution in cells or tissue. The present method provides a valuable tool for imaging phospholipid synthesis, turnover and subcellular localization in cultured cells as well as in animals.