The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 24, 2015
Filed:
Nov. 17, 2011
David M. Donovan, Baltimore, MD (US);
Lorena Rodriguez Rubio, Asturias, ES;
Beatriz Martinez Fernandez, Asturias, ES;
Ana Rodriguez, Asturias, ES;
Pilar Garcia Suarez, Asturias, ES;
David M. Donovan, Baltimore, MD (US);
Lorena Rodriguez Rubio, Asturias, ES;
Beatriz Martinez Fernandez, Asturias, ES;
Ana Rodriguez, Asturias, ES;
Pilar Garcia Suarez, Asturias, ES;
The United States of America, as represented by The Secretary of Agriculture, Washington, DC (US);
Abstract
Virion-associated peptidoglycan hydrolases have a potential as antimicrobial agents due to their ability to lyse Gram positive bacteria on contact. Full-length HydH5, a virion-associated peptidoglycan hydrolase from thebacteriophage vB_SauS-phi-IPLA88, and two truncated derivatives, containing only the CHAP domain, exhibited high lytic activity against livecells. Three different fusion proteins were created and showed higher staphylolytic activity than the parental enzyme or its deletion construct. Parental and fusion proteins lysedcells in zymograms, plate lysis and turbidity reduction assays. In plate lysis assays, HydH5 and its derivative fusions lysed bovine and humanMRSA N315 strain, and humanstrains. HydH5 and its derivative fusions proteins displayed antimicrobial synergy with the endolysin LysH5 in vitro suggesting that the two enzymes have distinct cut sites and thus may be more efficient in combination for the elimination of staphylococcal infections.