The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 24, 2015

Filed:

May. 11, 2012
Applicants:

Erhard Ralf Schoenbrunner, Moraga, CA (US);

Sven-erik Behrens, Halle, DE;

Inventors:

Erhard Ralf Schoenbrunner, Moraga, CA (US);

Sven-Erik Behrens, Halle, DE;

Assignee:

Life Technologies Corporation, Carlsbad, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01N 63/00 (2006.01); C12N 15/00 (2006.01); C07H 21/02 (2006.01); C12N 7/00 (2006.01); C12N 15/86 (2006.01);
U.S. Cl.
CPC ...
C12N 7/00 (2013.01); C12N 15/86 (2013.01); C07H 21/02 (2013.01); C12N 2770/24211 (2013.01); C12N 2770/24222 (2013.01); C12N 2770/24311 (2013.01); C12N 2770/24322 (2013.01);
Abstract

The invention relates to the field of nucleic acid amplification, particularly to quality control materials for use in viral RNA assays. It specifically relates to the construction of a recombinantby the identification of a region in the 3'NTR of the viral RNA genome where additional sequence elements can be stably inserted. Chimericwith sequence insertions in the 3′ nontranslated region (3′NTR) of the viral RNA genome were stable in replication and capable of forming infectious, RNase resistant virus particles. This chimericwith a 3′NTR insertion can be utilized as a quality control material in analytical assays for RNA targets, including external, internal controls, quantitative standards in PCR and NAT nucleic acid assays.


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