The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 17, 2015
Filed:
Jun. 30, 2010
Applicants:
Bakhos A. Tannous, Malden, MA (US);
Casey Maguire, Arlington, MA (US);
Inventors:
Bakhos A. Tannous, Malden, MA (US);
Casey Maguire, Arlington, MA (US);
Assignee:
The General Hospital Corporation, Boston, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
C12Y 113/12005 (2013.01); G01N 2333/90241 (2013.01); C07K 2319/00 (2013.01); C12N 9/0069 (2013.01);
Abstract
Described herein is a variant of wild type Gaussia luciferase that catalyzes glow-type emission kinetics suited for high-throughput functional screening applications. Polypeptides, functional fragments, variants, and nucleic acids that encode the enhanced luciferase are further described. One such polypeptide corresponds to wild type Gaussia luciferase with a substitution mutation of I for M at position 43 of the mature peptide. Methods of use, assay systems and kits that contain the polypeptides and/or nucleic acids are further described.