The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 10, 2015

Filed:

Oct. 18, 2010
Applicants:

Phillip D. Zamore, Northborough, MA (US);

Melissa J. Moore, Chestnut Hill, MA (US);

Christian Roy, Shrewsbury, MA (US);

Inventors:

Phillip D. Zamore, Northborough, MA (US);

Melissa J. Moore, Chestnut Hill, MA (US);

Christian Roy, Shrewsbury, MA (US);

Assignee:

University Of Massachusetts, Boston, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C07H 21/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); C12N 15/10 (2006.01); C12N 15/66 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1031 (2013.01); C12N 15/1096 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6869 (2013.01); C12N 15/66 (2013.01);
Abstract

A technology is described that is capable of generating high-throughput sequencing (HTS) read length DNA products to accurately and reliably provide exon connectivity information for alternatively spliced isoforms. The method is not limited by the initial size of the isoform as the technology removes the template oligonucleotide sequence and a newly formed full length ligated product provides an HTS-compatible read length sequence that comprises information that corresponds to the consecutive order of the exons in the original template oligonucleotide.


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