The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 03, 2015
Filed:
Jan. 22, 2014
Applicant:
Butamax Advanced Biofuels Llc, Wilmington, DE (US);
Inventors:
Larry Cameron Anthony, Aston, PA (US);
Lori Ann Maggio-Hall, Wilmington, DE (US);
Assignee:
Butamax Advanced Biofuels LLC, , DE (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/16 (2006.01); C12P 7/16 (2006.01); C12N 9/88 (2006.01); C12P 7/04 (2006.01);
U.S. Cl.
CPC ...
C12P 7/16 (2013.01); C12N 9/88 (2013.01); C12P 7/04 (2013.01); C12Y 202/01006 (2013.01); Y02E 50/10 (2013.01);
Abstract
A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains.