The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 03, 2015

Filed:

Mar. 22, 2012
Applicants:

Martin Stelzle, Reutlingen, DE;

Brigitte Angres, Pfullingen, DE;

Massimo Kubon, Muensingen, DE;

Julia Schuette, Tuebingen, DE;

Britta Hagmeyer, Tuebingen, DE;

Felix Holzner, Langnau am Albis, CH;

Inventors:

Martin Stelzle, Reutlingen, DE;

Brigitte Angres, Pfullingen, DE;

Massimo Kubon, Muensingen, DE;

Julia Schuette, Tuebingen, DE;

Britta Hagmeyer, Tuebingen, DE;

Felix Holzner, Langnau am Albis, CH;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D 57/02 (2006.01); C12M 3/06 (2006.01); C12M 3/00 (2006.01); C12M 1/42 (2006.01); C12M 1/26 (2006.01); B01L 3/00 (2006.01);
U.S. Cl.
CPC ...
C12M 23/16 (2013.01); C12M 21/08 (2013.01); C12M 35/02 (2013.01); C12M 33/00 (2013.01); B01L 3/5027 (2013.01);
Abstract

The present invention provides an apparatus and a method to assemble organotypical tissues which can then be perfused and investigated under preferably physiological conditions. This is achieved by a microfluidic system for assembling and subsequently cultivating complex cell arrangements, comprising: a three-dimensional microstructure in which the cell arrangement is assembled and cultivated, at least two microchannel segments running in the microstructure and defining a flow direction, through which microchannel segments the microstructure can be perfused from outside with a medium, whereby the microchannel segments run approximately parallel or equidistant to one another at least in sections, a wall structure which separates the at least two microchannel segments and in which at least one aperture connecting the at least two microchannel segments is provided, and an electrode arrangement provided in or on the microstructure in order to generate an inhomogeneous electric field in the region of the at least one aperture.


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