The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 24, 2015

Filed:

Apr. 03, 2012
Applicants:

Hyeon-je Cho, Fremont, CA (US);

Linda A. Castle, Mountain View, CA (US);

Theodore M. Klein, Wilmington, DE (US);

Tai Minh, San Jose, CA (US);

York Moy, San Francisco, CA (US);

Inventors:

Hyeon-Je Cho, Fremont, CA (US);

Linda A. Castle, Mountain View, CA (US);

Theodore M. Klein, Wilmington, DE (US);

Tai Minh, San Jose, CA (US);

York Moy, San Francisco, CA (US);

Assignees:

Pioneer Bred International Inc, Johnston, IA (US);

E.I. DuPont de Nemours, Wilmington, DE (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 15/82 (2006.01); C12N 1/20 (2006.01);
U.S. Cl.
CPC ...
C12N 15/8205 (2013.01); C12N 1/20 (2013.01);
Abstract

Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.


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