The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 24, 2015

Filed:

May. 11, 2007
Applicants:

Stephen Lovell, Sparks, MD (US);

Lydia Blank, Sparks, MD (US);

Virginia Crews, Sparks, MD (US);

Nancy Hasse, Sparks, MD (US);

Peter LU, Sunnyvale, CA (US);

Johannes Schweizer, Sunnyvale, CA (US);

John Mantlo, Westminster, MD (US);

Inventors:

Stephen Lovell, Sparks, MD (US);

Lydia Blank, Sparks, MD (US);

Virginia Crews, Sparks, MD (US);

Nancy Hasse, Sparks, MD (US);

Peter Lu, Sunnyvale, CA (US);

Johannes Schweizer, Sunnyvale, CA (US);

John Mantlo, Westminster, MD (US);

Assignees:

Arbor Vita Corporation, Sunnyvale, CA (US);

Becton, Dickinson and Company, Franklin Lakes, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/18 (2006.01); C07K 14/47 (2006.01); A61K 45/06 (2006.01); G01N 33/569 (2006.01);
U.S. Cl.
CPC ...
G01N 33/56983 (2013.01); G01N 2333/025 (2013.01);
Abstract

Methods for producing a protein extract from cells, such as cells containing viral proteins, are provided. In general terms, the methods involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent, neutralizing the pH of the intermediate composition to produce the protein extract. Kits and compositions for practicing the subject methods are also provided.


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