The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 24, 2015
Filed:
Oct. 20, 2010
Anatoly Kachurin, Orlando, FL (US);
Vaughan Wittman, Oviedo, FL (US);
Mike N. Nguyen, Orlando, FL (US);
Olga Kachurina, Orlando, FL (US);
Tenekua Tapia, Orlando, FL (US);
Vipra Dhir, Orlando, FL (US);
Alexander Karol, Orlando, FL (US);
Anatoly Kachurin, Orlando, FL (US);
Vaughan Wittman, Oviedo, FL (US);
Mike N. Nguyen, Orlando, FL (US);
Olga Kachurina, Orlando, FL (US);
Tenekua Tapia, Orlando, FL (US);
Vipra Dhir, Orlando, FL (US);
Alexander Karol, Orlando, FL (US);
Sanofi Pasteur Vaxdesign Corp., Orlando, FL (US);
Abstract
Hemagglutination (HA) and hemagglutination inhibition (HAI) functional assays remain important instruments of analysis of virus-cell interaction and protecting efficacy of virus-specific antibodies and sera. However, they demonstrate limited sensitivity towards many viruses, and require significant volumes of viruses, erythrocytes, sera, and antibodies. The present invention comprises new and significantly more sensitive versions of the HA and HAI assays based on observing agglutination on activated surfaces of specifically opsonized plates and ELISA plates rather than in solution. A version of the new assay that uses ELISA plates additionally allows characterizing the affinity of functional antibodies in the tested sera and fluids, which is not possible in the classical HAI assay. The methods of the present invention can also be used to improve the sensitivity of agglutination methods based on latex beads and to develop agglutination methods using target cells other than erythrocytes.