The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 24, 2015

Filed:

Jun. 24, 2013
Applicant:

Ibis Biosciences, Inc., Carlsbad, CA (US);

Inventors:

Mark Eshoo, San Diego, CA (US);

John Picuri, Carlsbad, CA (US);

Assignee:

IBIS Biosciences, Inc., Carlsbad, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12Q 1/00 (2006.01); B01L 3/00 (2006.01); C12N 9/96 (2006.01); B01L 7/00 (2006.01); B01L 9/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); B01L 3/5027 (2013.01); C12N 9/96 (2013.01); C12Q 1/6874 (2013.01); B01L 7/52 (2013.01); B01L 9/527 (2013.01); B01L 2200/10 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0672 (2013.01); B01L 2400/0481 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0655 (2013.01); B01L 2400/0683 (2013.01);
Abstract

The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods (e.g., next generation sequencing methods) or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme (e.g., an enzyme used in whole genome amplification), BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.


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