The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 17, 2015
Filed:
May. 06, 2011
Applicant:
James R. Swartz, Menlo Park, CA (US);
Inventor:
James R. Swartz, Menlo Park, CA (US);
Assignees:
GreenLight Biosciences, Inc., Medford, MA (US);
The Board of Trustees of the Leland Stanford Junior University, Palo Alto, CA (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/21 (2006.01); C12P 7/16 (2006.01); C12N 15/63 (2006.01); C12N 9/00 (2006.01); C12P 7/42 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12N 15/62 (2006.01); C12P 7/62 (2006.01); C12P 21/00 (2006.01); C12P 23/00 (2006.01); C12N 1/06 (2006.01); C12P 13/00 (2006.01); C12P 7/00 (2006.01); C12P 5/00 (2006.01);
U.S. Cl.
CPC ...
C12P 7/42 (2013.01); C07K 2319/034 (2013.01); C12N 9/1029 (2013.01); C12N 9/88 (2013.01); C12N 15/625 (2013.01); C12P 7/16 (2013.01); C12P 7/625 (2013.01); C12P 23/00 (2013.01); Y02E 50/10 (2013.01);
Abstract
Genetically manipulated cells, lysates of such cells, systems, and methods of use thereof are provided, where one or more enzymes in a pathway of interest are genetically modified to incorporate a peptide sequence that provides for relocation of the protein, e.g., to the periplasm, so as to sequester the enzyme, and where the enzyme controls flux in the pathway of interest.