The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 10, 2015

Filed:

Jun. 24, 2010
Applicants:

Paulo Cezar DE Lucca, Campinas, BR;

Shujie Dong, Cary, NC (US);

Robert Jason Christopher Geijskes, Brisbane, AU;

Erik Martin Dunder, Hillsborough, NC (US);

Manuel Benito Sainz, Ribeirao Preto, BR;

Inventors:

Paulo Cezar De Lucca, Campinas, BR;

Shujie Dong, Cary, NC (US);

Robert Jason Christopher Geijskes, Brisbane, AU;

Erik Martin Dunder, Hillsborough, NC (US);

Manuel Benito Sainz, Ribeirao Preto, BR;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A01H 1/02 (2006.01); C12N 15/82 (2006.01);
U.S. Cl.
CPC ...
C12N 15/8205 (2013.01);
Abstract

The present invention provides methods for producing a transformed sugar cane tissue or cell thereof, said methods comprising: a) inoculating a sugar cane tissue or a cell thereof with aninoculation suspension, saidcomprising a nucleic acid of interest, to obtain an-inoculated sugar cane tissue or cell thereof; b) co-cultivating said-inoculated sugar cane tissue or cell thereof on a surface in the absence of co-culture media for a time period sufficient to reduce original weight of said-inoculated sugar cane tissue or cell thereof; and c) selecting a transformed sugar cane tissue or a cell thereof comprising said nucleic acid of interest. The transformation methods of the invention provide for increased transformation frequency and recovery of transgenic sugar cane plants.


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