The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 10, 2015
Filed:
Aug. 25, 2009
Applicants:
Anna Domogatskaya, Rönninge, SE;
Sergey Rodin, Stockholm, SE;
Karl Tryggvason, Djursholm, SE;
Inventors:
Assignee:
BioLamina AB, Sundbyberg, SE;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/07 (2010.01); C12N 5/10 (2006.01); C12N 5/071 (2010.01); C12N 5/0735 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0606 (2013.01); C12N 2533/52 (2013.01);
Abstract
The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, that both maintain stem cells in vitro pluripotency and enable self-renewal. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or their functional domains, in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferated and maintained their pluripotency.