The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 10, 2015
Filed:
May. 23, 2012
Tung-ming Pan, Taipei, TW;
Chao-wen Lin, Puli Township, Nantou County, TW;
Kung-yuan Chang, Caotun Township, Nantou County, TW;
Min-hsien Wu, Kaohsiung, TW;
Shiao-wen Tsai, Taipei, TW;
Tung-Ming Pan, Taipei, TW;
Chao-Wen Lin, Puli Township, Nantou County, TW;
Kung-Yuan Chang, Caotun Township, Nantou County, TW;
Min-Hsien Wu, Kaohsiung, TW;
Shiao-Wen Tsai, Taipei, TW;
Chang Gung University, Taoyuan, TW;
Abstract
A detection method for a sensor membrane formed of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes includes adding 1.0 g of NIPAAm powder to 20 ml water, heating same at 60° C. to form NIPAAm solution, and cooling the NIPAAm solution; adding 200 μl of 98.7 wt % of APS and 50 μl of 99 wt % of TEMED to the NIPAAm solution, uniformly mixing same, and reacting the mixture for 30 hours to prepare a transparent, gel PNIPAAm; adding 5 mg enzymes to 100 μl of 1×PBS buffer solution, uniformly mixing same, adding 100 μl of PNIPAAm to the buffer solution, and uniformly mixing the buffer solution; placing a biosensor on a heater for heating at a constant temperature of 37° C. with the biosensor being an EIS sensor having a sensor membrane formed of EuTiO; and taking a measurement.