The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 03, 2015
Filed:
Nov. 12, 2013
Matthew Omon Herigstad, Charlestown, MA (US);
Linda E. Rich, Worcester, MA (US);
Stephen Ming-teh LU, Worcester, MA (US);
Natarajan Ramasubramanyan, Westborough, MA (US);
Matthew Omon Herigstad, Charlestown, MA (US);
Linda E. Rich, Worcester, MA (US);
Stephen Ming-teh Lu, Worcester, MA (US);
Natarajan Ramasubramanyan, Westborough, MA (US);
AbbVie Inc., North Chicago, IL (US);
Abstract
The present invention is directed to methods for purifying a protein of interest, e.g., an antibody, from a sample comprising the protein of interest and at least one impurity, e.g., an aggregate, by employing a hydrophobic interaction chromatography (HIC) method that allows for binding of both the protein of interest and the at least one impurity under strong binding conditions. The present invention is based, at least in part, on the finding that both flow through and bind-elute techniques can be combined to achieve greater purification and recovery of a protein of interest, e.g., an antibody, under isocratic wash conditions and strong binding conditions.