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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 8945922 B1

PDFFull Text
Date of Patent:
Feb. 03, 2015

Filed:

Sep. 08, 2009

Generating a mature nkt cell from a reprogrammed somatic cell with a t-cell antigen receptor α-chain region rearranged to uniform va-ja in a nkt-cell specific way

Applicants:

Hiroshi Watarai, Kanagawa, JP;

Haruhiko Koseki, Kanagawa, JP;

Masaru Taniguchi, Kanagawa, JP;

Shin-ichiro Fujii, Kanagawa, JP;

Inventors:

Hiroshi Watarai, Kanagawa, JP;

Haruhiko Koseki, Kanagawa, JP;

Masaru Taniguchi, Kanagawa, JP;

Shin-ichiro Fujii, Kanagawa, JP;

Assignee:

Riken, Wako, JP;

Attorney:

Leydig, Voit & Mayer, Ltd.

Primary Examiner:

Deborah Crouch

Int. Cl.
CPC ...
C12N 5/02 (2006.01); A61K 31/7036 (2006.01); C12N 5/0783 (2010.01); C12N 5/074 (2010.01); A61K 35/12 (2006.01);
U.S. Cl.
CPC ...
A61K 31/7036 (2013.01); A61K 35/12 (2013.01); C12N 5/0646 (2013.01); C12N 5/0696 (2013.01); C12N 2501/23 (2013.01); C12N 2501/2302 (2013.01); C12N 2501/2307 (2013.01); C12N 2501/2315 (2013.01); C12N 2501/26 (2013.01); C12N 2501/42 (2013.01); C12N 2501/60 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/604 (2013.01); C12N 2501/606 (2013.01); C12N 2506/11 (2013.01); C12N 2506/45 (2013.01);
Abstract

Provided are an iPS cell derived from a somatic cell such as an NKT cell, having the α-chain region of the T cell antigen receptor gene rearranged to uniform Vα-Jα in an NKT cell receptor-specific way, NKT cells differentiated from the iPS cell, a method of creating the same, and an immune cell therapy agent prepared using cells differentiated from the iPS cell. Also provided are an iPS cell having TCRα rearranged to NKT-TCR (NKT-iPS cell), obtained by contacting a somatic cell, such as an NKT cell, having the α-chain region of the T cell antigen receptor gene rearranged to uniform Vα-Jα in an NKT cell receptor-specific way, with nuclear reprogramming factors, isolated NKT cells obtained by differentiating the iPS cell ex vivo (iPS-NKT cell), a method of generating CD4/CD8-double positive NKT cells (DP-NKT cells) and mature NKT cells from NKT-iPS cells by altering the combination of feeder cells and/or cytokines, a method of expanding the iPS-NKT cells, and an NKT cell cytotherapy agent comprising NKT cells activated with α-galactosyl ceramide (α-GalCer), or iPS-NKT cells, and α-GalCer in combination.

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