The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 03, 2015

Filed:

Nov. 16, 2010
Applicants:

Joachim Jose, Düsseldorf, DE;

Ruth Maas, Düsseldorf, DE;

Christian Detzel, Duisburg, DE;

Inventors:

Joachim Jose, Düsseldorf, DE;

Ruth Maas, Düsseldorf, DE;

Christian Detzel, Duisburg, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/70 (2006.01); C12P 7/42 (2006.01); C12N 9/78 (2006.01);
U.S. Cl.
CPC ...
C12P 7/42 (2013.01); C12N 9/78 (2013.01); C12N 15/70 (2013.01); C07K 2319/034 (2013.01); C07K 2319/035 (2013.01);
Abstract

The present invention relates to a method for producing a product of a reaction catalysed by a nitrilase, which method comprises the steps (i) providing a microorganism comprising said nitrilase located on its surface, and/or a membrane preparation of said microorganism, and (ii) contacting the microorganism and/or the membrane preparation thereof with one or more nitrilase substrates under conditions compatible with nitrilase activity. The present invention further relates to a method for producing enantiomerically pure (R)-mandelic acid using the nitrilase-displaying whole cell biocatalyst or membrane preparation thereof for the conversion of racemic mandelonitrile.


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