The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 27, 2015

Filed:

Nov. 10, 2008
Applicants:

Eric A. Johnson, Madison, WI (US);

Sabine Pellett, Madison, WI (US);

William H. Tepp, Madison, WI (US);

Gary E. Borodic, Cambridge, MA (US);

David J. Beebe, Monona, WI (US);

Amy Paguirigan, Seattle, WA (US);

Inventors:

Eric A. Johnson, Madison, WI (US);

Sabine Pellett, Madison, WI (US);

William H. Tepp, Madison, WI (US);

Gary E. Borodic, Cambridge, MA (US);

David J. Beebe, Monona, WI (US);

Amy Paguirigan, Seattle, WA (US);

Assignees:

Wisconsin Alumni Research Foundation, Madison, WI (US);

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/00 (2006.01); C12Q 1/68 (2006.01); G01N 33/567 (2006.01); C12Q 1/37 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/37 (2013.01); G01N 2333/33 (2013.01);
Abstract

A highly sensitive method of analyzing a sample for the presence or activity of botulinum neurotoxin (BoNT) or antibodies specific for botulinum neurotoxin is disclosed. In one embodiment, the method comprises the steps of preparing primary non-human mammalian or avian spinal cord cells, and exposing the cells to a test sample, in parallel with a control sample, and examining the extent of cleavage of the intracellular neuronal target protein in both the test and control sample.


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