The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 13, 2015

Filed:

May. 04, 2007
Applicants:

Christian Korfhage, Langenfeld, DE;

Holger Engel, Hilden, DE;

Dirk Löffert, Düsselfdorf, DE;

Ralf Peist, Hilden, DE;

Inventors:

Christian Korfhage, Langenfeld, DE;

Holger Engel, Hilden, DE;

Dirk Löffert, Düsselfdorf, DE;

Ralf Peist, Hilden, DE;

Assignee:

Qiagen GmbH, Hilden, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/00 (2006.01); C12Q 1/68 (2006.01); C12P 19/34 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12N 15/10 (2013.01); C12Q 1/6816 (2013.01);
Abstract

The present invention concerns a method for inserting one or more tag sequences into a nucleic acid characterized by the following steps: (a) preparation of a template nucleic acid; (b) hybridization of at least one anchor sequence of at least one anchor oligonucleotide with one sequence section of the template nucleic acid; and (c) synthesization of a new strand of nucleic acid, which is partially complementary to the template nucleic acid and which contains a sequence complementary to the non-hybridized portion of the anchor oligonucleotide, e.g. to at least one tag sequence, on its 3' end.


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