The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 06, 2015

Filed:

Oct. 07, 2010
Applicants:

Cyrus K. Aidun, Marietta, GA (US);

Ulrika Egertsdotter, Marietta, GA (US);

Inventors:

Cyrus K. Aidun, Marietta, GA (US);

Ulrika Egertsdotter, Marietta, GA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/02 (2006.01); A61B 17/43 (2006.01); C12M 1/33 (2006.01);
U.S. Cl.
CPC ...
C12M 45/02 (2013.01);
Abstract

Methods and devices for dispersion of assemblies of biological material (such as plant embryogenic mass, plant tissue, cultured plant cells, animal tissue and/or cultured animal cells) suspended in a liquid are disclosed. The methods comprise i) subjecting the assemblies of biological material to fluid dynamics forces causing axially extensional strain and radially compressional strain and ii) subjecting the assemblies of biological material to fluid dynamics forces causing axially compressional strain and radially extensional strain fluid dynamics and iii) repeating said steps i) and ii) in sequence until assemblies of biological material is dispersed into the desired smaller size. The devices may comprise a flow channel arranged in a loop configuration for re-circulation in the flow channel, the flow channel including at least one constriction, such that the assemblies of biological material flowing through the flow channel are first subjected to axially extensional strain and radially compressional strain, and then to axially compressional strain and radially extensional strain from fluid dynamics forces.


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