The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 06, 2015
Filed:
Dec. 15, 2011
Gusti Zeiner, San Mateo, CA (US);
Robert A. Ach, San Francisco, CA (US);
Gusti Zeiner, San Mateo, CA (US);
Robert A. Ach, San Francisco, CA (US);
Agilent Technologies, Inc., Santa Clara, CA (US);
Abstract
Provided herein is a method of preparing an RNA sample comprising: a) obtaining an RNA sample comprising: i. long RNA molecules that may be unfragmented or fragmented to contain 5'-OH group and a 2′-3′-cyclic phosphate group; and ii. short RNA molecules that comprise a 5′ phosphate group and a 3′ OH group; and b) contacting the RNA sample with an adaptor comprising either a 2′-PO group and 3′-OH group or a 2′,3′-cyclic phosphate group in the presence of a eukaryotic tRNA ligase, thereby producing a ligated RNA sample in which a) the short RNA molecules are selectively ligated to the adaptor or b) the short RNA molecules and long RNA fragments are selectively ligated to the adaptor.