The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 30, 2014
Filed:
Jun. 05, 2012
Yen-pei LU, Hsinchu, TW;
Ming-yu Lin, Hsinchu, TW;
Yu-sheng Lai, Zhudong Township, TW;
Yuh-shyong Yang, Hsinchu, TW;
Hsuen-li Chen, Taipei, TW;
Yu-cheng Ou, Zhunan Township, TW;
Yen-Pei Lu, Hsinchu, TW;
Ming-Yu Lin, Hsinchu, TW;
Yu-Sheng Lai, Zhudong Township, TW;
Yuh-Shyong Yang, Hsinchu, TW;
Hsuen-Li Chen, Taipei, TW;
Yu-Cheng Ou, Zhunan Township, TW;
National Applied Research Laboratories, Taipei, TW;
Abstract
A method of manufacturing a nanoparticle chain is disclosed. The method comprises the steps of: providing a single-stranded circular primer with a determined length, and amplifying the single-stranded circular primer into single-stranded DNA nanotemplate by an isothermal nucleotide amplification reaction such that an end of the single-stranded DNA nanotemplate is fixed to a surface of a substrate; and adding a single-stranded DNA probe conjugated with nanoparticle at one end of which, and attaching the single-stranded DNA probe to the corresponding sequence on the single-stranded DNA nanotemplate to form a nanoparticles chain. The method of manufacturing a nanoparticle chain further comprises providing a fluid, and the flowing direction of the fluid controls the aligning direction of the nanoparticle chain. Wherein, the inter-nanoparticle distance of the nanoparticle chain can be adjusted by adjusting a reaction temperature or adding the single-stranded DNA probe without conjugating with nanoparticles.