The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 23, 2014

Filed:

Oct. 10, 2006
Applicants:

Scott D. Boden, Atlanta, GA (US);

Sreedhara Sangadala, Dallas, GA (US);

F. Louisa Titus, Atlanta, GA (US);

William F. Mckay, Memphis, TN (US);

Inventors:

Scott D. Boden, Atlanta, GA (US);

Sreedhara Sangadala, Dallas, GA (US);

F. Louisa Titus, Atlanta, GA (US);

William F. McKay, Memphis, TN (US);

Assignee:

Warsaw Orthopedic, Inc., Warsaw, IN (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 1/36 (2006.01); C07K 1/22 (2006.01); C07K 14/435 (2006.01); C07K 16/18 (2006.01); C07K 14/78 (2006.01); A61L 27/38 (2006.01); C07K 14/47 (2006.01); A61K 48/00 (2006.01); A61K 35/12 (2006.01); A61K 35/00 (2006.01);
U.S. Cl.
CPC ...
A61L 27/3856 (2013.01); C07K 16/18 (2013.01); C07K 14/78 (2013.01); A61K 2121/00 (2013.01); A61K 48/00 (2013.01); A61K 35/12 (2013.01); C07K 14/47 (2013.01); A61K 35/13 (2013.01); C12N 2799/022 (2013.01);
Abstract

Methods of expressing LIM mineralization protein in mammalian cells are described. Methods of expressing LIM mineralization protein and assessing glycosylation of the LIM mineralization protein in prokaryotic and non-mammalian eukaryotic cells are also described. The methods involve transfecting the cells with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein. Transfection may be accomplished in vitro, ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid.


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