The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 23, 2014

Filed:

Feb. 17, 2005
Applicants:

Kambiz Shekdar, New York, NY (US);

Dennis J. Sawchuk, Hoboken, NJ (US);

Jason M. Montez, Rumson, NJ (US);

Inventors:

Kambiz Shekdar, New York, NY (US);

Dennis J. Sawchuk, Hoboken, NJ (US);

Jason M. Montez, Rumson, NJ (US);

Assignee:

Chromocell Corporation, North Brunswick, NJ (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C40B 20/00 (2006.01); C12Q 1/68 (2006.01); C12N 15/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6841 (2013.01); C12Q 2525/207 (2013.01); C12Q 2565/1015 (2013.01); C12Q 1/6841 (2013.01); C12Q 2525/301 (2013.01);
Abstract

Methods of isolating cells or generating cell lines comprising the step of exposing the cells to signaling probes that produce a signal upon hybridization to a target sequence, as well as methods of quantifying the level of expression of an RNA of interest, methods for identifying genetic recombinational events in living cells and methods of generating a transgenic animal using the isolated cells. Methods for isolating a plurality of cells encoding a plurality of different RNAs associated with a same nucleic acid tag sequence, comprising the step of exposing the cells to a same signaling probe that produces a detectable signal upon hybridization to the same nucleic acid tag sequence, are also provided. Signaling probes and protease probes that form stem-loop structures, three-arm junction structures, and dumbbell structures may be used in the above methods.


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