The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 23, 2014

Filed:

Dec. 06, 2011
Applicants:

John J. Naleway, Eugene, OR (US);

Ying Jiang, Eugene, OR (US);

Ryan Link-cole, Monmouth, OR (US);

Inventors:

John J. Naleway, Eugene, OR (US);

Ying Jiang, Eugene, OR (US);

Ryan Link-Cole, Monmouth, OR (US);

Assignee:

Marker Gene Technology, Inc., Eugene, OR (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C07D 209/24 (2006.01); C07D 471/04 (2006.01); C07D 495/04 (2006.01); C07J 19/00 (2006.01); C07D 311/16 (2006.01); C07D 219/14 (2006.01); C07D 271/12 (2006.01); C07D 471/06 (2006.01); C07D 403/06 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6841 (2013.01); C07D 471/04 (2013.01); C07D 495/04 (2013.01); C07J 19/00 (2013.01); C07D 209/24 (2013.01); C07D 311/16 (2013.01); C07D 219/14 (2013.01); C07D 271/12 (2013.01); C07D 471/06 (2013.01); C07D 403/06 (2013.01);
Abstract

The present invention provides systems and methods for production of activatable diazo-derivatives for use in labeling nucleotides. Labeling nucleotides is accomplished by contacting a stable hydrazide derivative of a detectable moiety with an activating polymer reagent which is used to directly label the nucleotide sample. Labeling occurs on the phosphate backbone of the nucleotide which does not perturb hybridization of the labeled nucleotide with its anti-sense strand. Since the method involves direct labeling, all types of nucleotides can be labeled without prior amplification or alteration.


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