The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 16, 2014

Filed:

Dec. 14, 2010
Applicants:

Mark Chaisson, San Francisco, CA (US);

Sonya Clark, Oakland, CA (US);

Christopher Silk, Oakland, CA (US);

John Lyle, Redwood Shores, CA (US);

Lei Jia, Palo Alto, CA (US);

Keith Bjornson, Union City, CA (US);

Inventors:

Mark Chaisson, San Francisco, CA (US);

Sonya Clark, Oakland, CA (US);

Christopher Silk, Oakland, CA (US);

John Lyle, Redwood Shores, CA (US);

Lei Jia, Palo Alto, CA (US);

Keith Bjornson, Union City, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); C12Q 1/6869 (2013.01); C12Q 2521/101 (2013.01); C12Q 2561/113 (2013.01); C12Q 2563/107 (2013.01);
Abstract

Systems and methods are provided for single-molecule sequencing of template nucleic acids in which the signal from a label attached to a polymerase enzyme is used to monitor conformational changes in the polymerase which occur while labeled nucleotides or nucleotide analogs are added to a growing nucleic acid chain which is complementary to the template nucleic acid. The signal indicative of the conformational state of the enzyme is used to determine with higher confidence when true nucleotide or nucleotide analog incorporation events occur, allowing for the improved quality of base calls and sequence determination.


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