The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 16, 2014

Filed:

Dec. 15, 2011
Applicants:

Yi Chen, Shanghai, CN;

Shidong Liu, Shanghai, CN;

Zhaoli Zhang, Shanghai, CN;

Chunxia Wang, Shanghai, CN;

Jing Kang, Shanghai, CN;

Xiaoming Ji, Shanghai, CN;

Inventors:

Yi Chen, Shanghai, CN;

Shidong Liu, Shanghai, CN;

Zhaoli Zhang, Shanghai, CN;

Chunxia Wang, Shanghai, CN;

Jing Kang, Shanghai, CN;

Xiaoming Ji, Shanghai, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/04 (2006.01); C07K 7/54 (2006.01); C12R 1/645 (2006.01); C12N 15/01 (2006.01); C12N 1/14 (2006.01); C07K 7/56 (2006.01);
U.S. Cl.
CPC ...
C07K 7/54 (2013.01); C12R 1/645 (2013.01); C12N 15/01 (2013.01); C12N 1/14 (2013.01); C07K 7/56 (2013.01);
Abstract

High-yield antibiotics producing strain, preparation method and use thereof are provided in the present invention. The high-yield strain is a mutagenized strain derived from, and deposited in CGMCC with the accession number of CGMCC 4129. The preparation method comprises the following steps: (a) mixing a seed liquid ofof Accession No. FERM BP-2635 with nitrosoguanidine to obtain a mixture a; (b) mixing said mixture a with a wall-breaking enzyme to obtain protoplasts; (c) regenerating said protoplasts to obtain single colonies; and (d) culturing said single colonies to obtain said mutagenized strain. The obtained strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable for industrialization.


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