The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 8911947 B1

PDF
Full Text
Expired
Date of Patent:
Dec. 16, 2014

Filed:

Aug. 28, 2007
Applicants:

Kazuhiko Nakatani, Suita, JP;

Fumie Takei, Suita, JP;

Masaki Hagihara, Suita, JP;

Inventors:

Kazuhiko Nakatani, Suita, JP;

Fumie Takei, Suita, JP;

Masaki Hagihara, Suita, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6858 (2013.01); C12Q 1/686 (2013.01); C12Q 2600/156 (2013.01);
Abstract

A method is provided which enables quick, convenient, inexpensive, and high sensitivity confirmation of nucleic acid amplification after a nucleic acid amplification reaction. The DNA fragment in accordance with the present invention is a single-stranded DNA fragment containing a hairpin structure which in turn contains a bulge, wherein the DNA fragment is used as being attached to the 5' end of a primer used in nucleic acid amplification. The nucleic acid amplification confirmation method in accordance with the present invention quantifies a hairpin primer containing the DNA fragment at its 5′ end by using bulge-binding fluorescent molecules after carrying out PCR or like nucleic acid amplification reaction using the hairpin primer. SNPs are detected quickly and conveniently at low cost and with high sensitivity by applying the nucleic acid amplification confirmation method, for example, to allele specific PCR.


Find Patent Forward Citations

Nakatani, Kazuhiko. (2014). Dna fragment used as attached to 5' end of primer used in nucleic acid amplification reaction and use of dna fragment (U.S. Patent No. 8911947). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/8911947

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