The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 16, 2014

Filed:

May. 24, 2007
Applicants:

Yuko Shimatani, Nagano, JP;

Yoshinori Hamaji, Hyogo, JP;

Hitomi Matsuhashi, Nagano, JP;

Jun Amano, Nagano, JP;

Shun'ichiro Taniguchi, Nagano, JP;

Minoru Fujimori, Ibaraki, JP;

Inventors:

Yuko Shimatani, Nagano, JP;

Yoshinori Hamaji, Hyogo, JP;

Hitomi Matsuhashi, Nagano, JP;

Jun Amano, Nagano, JP;

Shun'ichiro Taniguchi, Nagano, JP;

Minoru Fujimori, Ibaraki, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01N 63/00 (2006.01); C12N 1/20 (2006.01); C12N 15/74 (2006.01); A61K 31/513 (2006.01); A61K 35/74 (2006.01); C12N 9/78 (2006.01); A61K 48/00 (2006.01);
U.S. Cl.
CPC ...
A61K 48/0008 (2013.01); A61K 48/00 (2013.01); A61K 31/513 (2013.01); A61K 35/745 (2013.01); A61K 35/74 (2013.01); C12N 9/78 (2013.01);
Abstract

An object of the present invention is to provide a method of efficiently constructing a gene delivery carrier having a favorable activity and expression efficiency of a protein expressed by a gene introduced by transformation. Moreover, an object of the present invention is to provide a pharmaceutical composition comprising a gene delivery carrier constructed by the construction method and a therapeutic agent for solid tumor comprising the resistant bacterium. The objects of the present invention have been attained by a gene delivery carrier consisting of an anaerobic microorganism capable of growing in a tumor tissue in an anaerobic environment and capable of expressing (A) a protein having an antitumor activity or (B) a protein having an activity of converting an antitumor substance precursor to an antitumor substance, wherein DNA encoding the protein of interest is allowed to further comprise, on the 5'-terminal side thereof, a DNA fragment comprising a nucleotide sequence encoding a peptide consisting of an amino acid sequence from the 1st to at least 4th amino acids at the N-terminus of a histone-like DNA-binding protein.


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