The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 09, 2014

Filed:

Jan. 12, 2009
Applicants:

Brett A. Chromy, Danville, CA (US);

Paul Henderson, Dublin, CA (US);

Paul D. Hoeprich, Jr., Pleasanton, CA (US);

Inventors:

Brett A. Chromy, Danville, CA (US);

Paul Henderson, Dublin, CA (US);

Paul D. Hoeprich, Jr., Pleasanton, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/00 (2006.01); C12P 3/00 (2006.01); C12N 11/02 (2006.01);
U.S. Cl.
CPC ...
C12P 3/00 (2013.01); C12N 11/02 (2013.01); Y10S 977/799 (2013.01);
Abstract

Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.


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