The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 09, 2014

Filed:

Mar. 30, 2009
Applicants:

John Eid, San Francisco, CA (US);

Stephen Turner, Menlo Park, CA (US);

Ravi Dalal, Menlo Park, CA (US);

Benjamin Flusberg, Palo Alto, CA (US);

Jonas Korlach, Newark, CA (US);

Steven Lin, Palo Alto, CA (US);

Adrian Fehr, Los Altos, CA (US);

Fred Christians, Los Altos Hills, CA (US);

Robin Emig, Belmont, CA (US);

Jeremy Gray, San Francisco, CA (US);

Robert Sebra, San Francisco, CA (US);

Lei Sun, San Jose, CA (US);

Stephen Dudek, San Francisco, CA (US);

Inventors:

John Eid, San Francisco, CA (US);

Stephen Turner, Menlo Park, CA (US);

Ravi Dalal, Menlo Park, CA (US);

Benjamin Flusberg, Palo Alto, CA (US);

Jonas Korlach, Newark, CA (US);

Steven Lin, Palo Alto, CA (US);

Adrian Fehr, Los Altos, CA (US);

Fred Christians, Los Altos Hills, CA (US);

Robin Emig, Belmont, CA (US);

Jeremy Gray, San Francisco, CA (US);

Robert Sebra, San Francisco, CA (US);

Lei Sun, San Jose, CA (US);

Stephen Dudek, San Francisco, CA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/48 (2006.01); C12N 11/14 (2006.01); G01N 33/543 (2006.01); C40B 20/02 (2006.01); C40B 60/08 (2006.01); C40B 40/06 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/54313 (2013.01); G01N 33/54366 (2013.01); C12N 2310/50 (2013.01); C40B 40/06 (2013.01); C12Q 1/6869 (2013.01); C12Q 2535/122 (2013.01); C12N 2310/51 (2013.01);
Abstract

Methods for non-random loading of single analyte molecules into array structures are provided. The methods allow for distribution of a population of target molecules into a plurality of size confined regions such as wells. Sizing moieties are linked to individual target molecules. The sizing moieties are of sufficient size, relative to the size-confined reaction or observation regions, such that only a selected number of sizing moieties will fit into the size confined regions. The confined regions and the sizing moieties or target molecules comprise a selected charge that allow for controlling the loading of the sizing moities.


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