The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 09, 2014

Filed:

Jul. 28, 2010
Applicants:

Hitoshi Matsui, Tokyo, JP;

Yasuyuki Sakai, Tokyo, JP;

Teruo Fujii, Tokyo, JP;

Shoji Takeuchi, Tokyo, JP;

Yukiko Tsuda, Tokyo, JP;

Inventors:

Hitoshi Matsui, Tokyo, JP;

Yasuyuki Sakai, Tokyo, JP;

Teruo Fujii, Tokyo, JP;

Shoji Takeuchi, Tokyo, JP;

Yukiko Tsuda, Tokyo, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 21/64 (2006.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01); C12M 1/00 (2006.01); C12N 5/071 (2010.01); C12M 1/04 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12M 23/20 (2013.01); C12N 2533/54 (2013.01); C12M 25/14 (2013.01); C12N 2533/30 (2013.01); C12M 41/46 (2013.01); C12N 5/0671 (2013.01); C12M 23/24 (2013.01); C12N 2503/00 (2013.01); G01N 33/5044 (2013.01);
Abstract

A method for culturing hepatocytes, wherein hepatocytes embedded in an extracellular matrix is placed on a gas-permeable membrane and the hepatocytes are cultured while being supplied with oxygen from the gas-permeable membrane side. By this, the polarity in the hepatocytes can be induced and a bile canaliculus can be formed in a short period of time. Further, the formed polarity can be maintained for a longer period.


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