The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 02, 2014

Filed:

Jul. 06, 2012
Applicants:

Allen E. Eckhardt, Durham, NC (US);

Jonathan Benton, Apex, NC (US);

Deborah Boles, Cary, NC (US);

Inventors:

Allen E. Eckhardt, Durham, NC (US);

Jonathan Benton, Apex, NC (US);

Deborah Boles, Cary, NC (US);

Assignee:

Advanced Liquid Logic, Inc., Research Triangle Park, NC (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C40B 30/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01); G06F 19/22 (2011.01); G06F 19/24 (2011.01);
U.S. Cl.
CPC ...
C12N 15/1089 (2013.01); C12Q 1/6869 (2013.01); G06F 19/22 (2013.01); G06F 19/24 (2013.01);
Abstract

The systems and methods of the invention provide a guided approach to pyrosequencing (i.e., hybrid pyrosequencing). A de novo nucleic acid sequence may compared to a library of possible results and the next nucleotide to be dispensed is selected based on the comparison of the de novo sequence and the library of possible results. In another example, at least the first nucleotide to be dispensed is selected based on a query of a database(s) of non-sequence parameters (e.g., incidence of infection, diagnostic symptoms, sample source) and subsequent dispensations determined based on a comparison of the de novo sequence and the library of possible results (e.g., candidate sequences). The systems and methods of the invention may be performed using a droplet actuator.


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