The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 02, 2014
Filed:
Nov. 30, 2009
Applicant:
Shih-chieh Hung, Taipei, TW;
Inventor:
Shih-Chieh Hung, Taipei, TW;
Assignee:
National Yang-Ming University, Taipei, TW;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/07 (2010.01); C12N 5/10 (2006.01); C12N 5/071 (2010.01); C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/0775 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0663 (2013.01); C12N 2500/02 (2013.01);
Abstract
The present invention relates to a novel method for expanding mesenchymal stem cells (MSCs) in low-density and hypoxic condition as compared to normal air conditions traditionally used in cell culture. The present method provides rapid and efficient expansion of human MSCs without losing cellular proliferation and stem cell properties, including increase in proliferation, decrease in senescence, and increase in differentiation potential both in vitro and in vivo. The expanded MSCs by the present method may maintain normal karyotyping, and will not form tumor when transplanted into mammal.