The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 25, 2014
Filed:
Aug. 08, 2011
Applicant:
Larry J. Cummings, Pleasant Hill, CA (US);
Inventor:
Larry J. Cummings, Pleasant Hill, CA (US);
Assignee:
Bio-Rad Laboratories, Inc., Hercules, CA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A23J 1/00 (2006.01); B01D 15/38 (2006.01); B01D 15/36 (2006.01); C07K 1/22 (2006.01); C07K 1/20 (2006.01); C07K 1/18 (2006.01); B01D 15/42 (2006.01);
U.S. Cl.
CPC ...
C07K 1/18 (2013.01); B01D 15/3804 (2013.01); B01D 15/426 (2013.01); B01D 15/361 (2013.01); C07K 1/22 (2013.01); C07K 1/20 (2013.01);
Abstract
Proteins, including monoclonal antibodies, that have been retained on hydroxyapatite resins for purposes of protein separation, purification, or both, are eluted from the resins by a elution buffer that contains controlled amounts of calcium and phosphate ions. The buffer allows elution to be performed in repeated runs at an acidic pH without deterioration of the resin.